Hello everyone, I was wondering whether anyone has had such an experience!!!
I loaded a 35 mg/ml concentrated protein (in a buffer containing 50 mM Arg and 50 mM Glu) for SEC in Superdex 200. I get a nice peak ~ 300mAu and have a volume 12 ml consisting of a few fractions included in the peak. However I cannot see anything in the coomasie gel when I load 20 uL of the fractions from the peaks. I concentrated the protein to ~ 20-25 mgs/ml and ran the gel and still nothing. The absorbance at 280 is high with a nice peak, but I can't see any band in the gel. Has it happened to anyone? Any suggestions or comments will be invaluable as always!!! Would silver staining help? Thanks in advance, Ivan
