It looks like I'm seeing density for both conformations I just wasn't sure how to build both in Coot. (Or in another program if necessary.)
On Thu, Feb 12, 2015 at 3:18 PM, Dale Tronrud <[email protected]> wrote: > -----BEGIN PGP SIGNED MESSAGE----- > Hash: SHA1 > > > I'm a little confused. If you see from the density that a peptide > flip is required, can't you just fix it? Or is it the case that the > density is telling you that both conformations are present? In that > case build alternative conformations and refine the occupancy. > > Dale Tronrud > > On 2/12/2015 9:07 AM, Kimberly Stanek wrote: > > Hello all, > > > > I am in some of the final stages of refinement of a 1.5 A > > resolution oligomeric protein and I'm noticing what appears to be > > extra density corresponding to a carbonyl peptide flip near the > > N-terminus of one of the chains. I was wondering if anyone had any > > experience with this before and if so, could point me to any > > relevant literature or references. > > > > Thanks, Kim > > > > -- Kimberly Stanek Graduate Student Mura Lab Department of > > Chemistry University of Virginia (434) 924-7979 > -----BEGIN PGP SIGNATURE----- > Version: GnuPG v2.0.22 (MingW32) > > iEYEARECAAYFAlTdCpQACgkQU5C0gGfAG13+AACgmzI4srRRK51d/dTUTYCggNK5 > EtUAoIkMS5lqN3+wrM3RVyPL2plQPjc9 > =/wOR > -----END PGP SIGNATURE----- > -- Kimberly Stanek Graduate Student Mura Lab Department of Chemistry University of Virginia (434) 924-7979
