> On Feb 26, 2015, at 4:51 PM, Srivastava, Dhiraj <[email protected]> > wrote: > > Hi > I have a protein with two substrate. when I am doing the binding studies > with the two substrate separately, I am finding one of the substrate to have > similar kd and Km. however the km and kd values are almost 30 fold different > for the other substrate. it binds 30 fold more tightly then you can think > based on Km. I don't know how to explain this. Km and Kd can be different but > does any one have seen that much difference in Kd and Km? > > > Thank you > Dhiraj
If k+2 isn’t small compared to k-1, Km cannot be approximated by Kd.
