Hi all, I have a data set from a large room-temperature lysozyme crystal consisting of 6 90-degree sweeps, each taken from a fresh spot on the crystal. When I scale & merge the first sweep by itself, the R[meas/merge] vs. resolution trace as reported by aimless looks fairly normal (lowest values in the 6-2.5 A range and steadily increasing at higher resolution). However, as I look at the subsequent sweeps individually, the traces get progressively stranger until the final sweep's R vs. resolution trace looks like a smiley face, lowest around 2.5 A and about the same in the lowest and highest resolution bins. The overall Rmeas is about the same for all sweeps (3.0-3.2%). Do you have any ideas on what has happened (either during data collection or data processing) to cause this?
Thanks, Veronica
