What about 4deg data collection? What about glutaraldehyde crosslinking?
JPK From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Ursula Schulze-Gahmen Sent: Monday, March 02, 2015 1:49 PM To: [email protected] Subject: [ccp4bb] cryo protection for low salt crystallization at 4 degrees I know there was jut recently a discussion about cryoconditions for crystals, but I am still hoping for some new ideas for my crystals that grow from HEPES buffer pH 7.3, 0.2 M NaCl by slowly lowering the temperature from 20 to 4 degrees. These crystals are easy to grow but extremely sensitive to temperature change, and any of the usual cryo reagents tested so far simply dissolve the crystals. I am n ot sure how I could counteract the solubilizing effect of glycerol or ethylen glycol, since there is no precipitant concentration that I could increase to stabilize the crystals. Paratone didn't work either so far. Any other ideas for these low salt crystals? Ursula -- Ursula Schulze-Gahmen, Ph.D. Project Scientist UC Berkeley, QB3 360 Stanley Hall #3220 Berkeley, CA 94720-3220 (510) 643 9491
