The SDS PAGE estimation can be done plus there are some nice glycoportein stains (periodic acid schiffs staining) could be used (if Coomasie staining is a problem) I do not know if the PAS staining is linear to standard staining. but regular staining should work like a charm use bovine rhodopsin or some other relatively easier proteins that can be used as a regular standards to compare and estimate the unkown sample concentration
For many membrane protein samples at the last stages of purification have interference from ligands and other buffer constituents are difficult to use absorption Padayatti On Tue, Mar 10, 2015 at 2:49 PM, Gmail <[email protected]> wrote: > Dear all, > > > > I have a (heavily and heterogeneously) glycosylated protein that has > very low OD280 absorption. I wondered whether the glycans will have any > effect on the accuracy of BCA or Bradford assay. Amino acid analysis > doesn’t seem to be any better because carbohydrates will interfere with > sample analysis and quantification. Any suggestion about other methods that > we could try to measure the concentration of this protein? Much appreciated! > > > > Best, > > Tianyu > > > -- P
