Hi all, Recently I am working on a protein and the phase could be obtained from low resolution datasets (~6A) of native and heavy atom derivative. Later a relatively higher native dataset with ~4A was obtained , but it is somewhat non-isomorphous to the previous datasets used for phasing, making it not suitable for SIR phasing with previous 6A heavy atom dataset.
After reading some literature, I realized that DMMULTI might work in my case to extend the phase to the higher resolution non-isomorphous dataset. However, I was not able to run the program successfully. Briefly, I provided the MTZ file of the initial low-resolution map then MTZ file of high-resolution native dataset, inputting the required field like solvent content, leaving other parameters as default. According to the log file, the program was able to go through the initial map MTZ file, however, when the program try to open MTZ file of the high resolution dataset, it gave me error message "CCP4 library signal library_file:Cannot open file". What is the possible reason for this? Do I need more inputs other than the high-resolution dataset and initial map? Thanks in advance,
