On 03/19/2015 07:20 AM, Smith Liu wrote:
Suppose I have a PDB file and a mtz file (PDB file from protein A, mtz
file from protein B, which is a homology protein of protein A) which
are not from the same refinment (thus not fit automatically in Coot),
will you please tell me what modification I should make on the files
in order to have the Coot to fit the protein bakbone (and the
sidechains) and the electron density?
Hi Smith ...
Open your protein-A-ref1.pdb file (File/Open Coordinates...) and
protein-A-ref1.mtz file (File/Auto Open MTZ ...),
then open your homologous protein-B.pdb and superpose it with
Calculcate/SSM Superpose ...
or if you are not happy with the result you can try to improve the
superposition with Calculate/LSQ Superpose ...
Best regards
Carlos
I am looking forward to getting your reply.
Smith
--
Carlos CONTRERAS MARTEL, Ph.D.
(CR1 CNRS)
[email protected]
"Bacterial Pathogenesis Group"
Institut de Biologie Structurale
UMR5075 CEA-CNRS-Université Joseph Fourier Grenoble 1
IBS
71, avenue des Martyrs
CS 10090
38044 Grenoble CEDEX 9
FRANCE
tel : (+33) (0)4 57 42 86 41
http://www.ibs.fr/groupes/groupe-pathogenie-bacterienne/?lang=fr
http://www.ibs.fr/groups/bacterial-pathogenesis-group/?lang=en
