Hello everyone. I have two queries regarding structure refinement:
1. I have a structure solved through SAD phasing. The density of chain A from residues 50-60 is good. However, the same positions have very less density in chain B and chain C. Can anyone suggest how I can build the positions 50-60 in chain B and C. Can I somehow replace these residues with the built chain A residues? If yes, then how? 2. I want to change the occupancy of about 50 residues dispersed across the chains to 0.5. Other than changing the occupancy manually in the pdb, is there any other option? Waiting for responses. Thanks -- Nazia Nasir, PhD Protein Crystallography Lab National Institute of Immunology New Delhi
