Dear all, I was trying to generate a one-dimensional electron density profile along the Z axis from an anomalous difference map (similar to Fig. 1 of Zhou, Y., & Mackinnon, R. (2003), Journal of Molecular Biology, 333(5), 965–975) using the program MAPMAN, but I think I’m missing something.
This is what I tried: - I’ve generated the anomalous difference map covering all the atoms in the pdb file using FFT from the CCP4 gui, doubling the grid space. - I fed MAPMAN with that anomalous map and ran the command “1d mymap”. - I then selected the Z axis. - I converted the O2D plot file into a PS file. But I think what I’m getting is only noise. Is there a way to plot the profile only for signals above 4 sigma? Cheers, Chris
