Isn't the only criteria for EDS that calculated R (by EDS) is +/- 5% of reported R?
On Thu, Apr 23, 2015 at 12:35 PM, Misbah ud Din Ahmad <[email protected] > wrote: > Dear Gert, > I just did that and I wonder how these structures end there !!! > > @ Sabine: I was talking about Ramachandran outliers. I had learnt that the > number of Ramachandran outliers show be very few or none for low resolution > structures. > > On Thu, Apr 23, 2015 at 6:25 PM, Gert Bange < > [email protected]> wrote: > >> Dear Mishba, >> >> Just check density vs model by simply open 'Coot', >> >> Go to: 'File' -> 'Fetch PDB & Map using EDS' >> >> Type the pdb entry into the field - enjoy the densities. >> >> Best and god save the EDS, >> >> Gert >> >> >> ----------------------------------------------------- >> LOEWE Center for Synthetic Microbiology >> AG Bange - Analysis of Metabolic Networks >> Dr. Gert Bange >> Hans-Meerwein-Strasse, C7 >> 35043 Marburg, Germany >> office: +49-6421-28-23361 >> fax: +49-6421-28-24430 >> web: www.synmikro.com/bange >> ----------------------------------------------------- >> LOEWE Center for Synthetic Microbiology >> AG Bange - Analysis of Metabolic Networks >> Dr. Gert Bange >> Hans-Meerwein-Strasse, C7 >> 35043 Marburg, Germany >> office: +49-6421-28-23361 >> fax: +49-6421-28-24430 >> web: www.synmikro.com/bange >> >> >> >> Am 23.04.2015 um 18:03 schrieb Misbah ud Din Ahmad <[email protected] >> >: >> >> Dear crystallographers, >> >> The PDB entry >> http://www.rcsb.org/pdb/explore.do?structureId=3BDN >> has 16.5% Ramachandran outliers. When I opened this PDB file in coot and >> checked for Ramachandran outliers, the results are: >> In preffered region: 58.04% >> In allowed regions: 19.78% >> >> *Outliers: 22.17% !!!!!!!!!* >> >> With an R-free of 37.4% at 3.9 A resolution, could you please tell me how >> reliable this structure of Lambda repressor bound to DNA is? >> >> >> Thanks >> Misbha >> >> >> >> >> >> >> >> >> >
