Also collect while rotating around the long axis, which you nearly are in this shot assuming the Phi axis is horizontal. That way the close separation is in x,y where you can see it rather than in Z which puts the spots on top of each other requiring thin slicing and (low mosaicity!) to separate.
eab On 04/26/2015 11:00 AM, Roger Rowlett wrote:
Have you tried increasing the camera distance (with a theta swing to get high res data) to get better spot separation? We've collected data sets with >300A edges this way. Thin slicing will help but won't perform miracles with a long cell edge and significant mosaicity. Roger Rowlett On Apr 26, 2015 10:03 AM, "weifei" <[email protected] <mailto:[email protected]>> wrote: Dear all, I have get a new crytsal but there is some difficulties in data collection. The predicted unit cell is large and all the molecules will be around the longest axis. The diffraction data was difficult to collect because the dots are too close to separate. I have try to do fine slicing but the result seems not better. Is anyone can give me some advice? Thank you so much! Sincerely, Weifei Chen
