Thanks a lot for your suggestions. Regards, Prerana
On Fri, Apr 24, 2015 at 7:05 PM, Tanner, John J. <[email protected]> wrote: > Following up on Dave's suggestion, you could try using crystal screens as > additives. This has worked well in my lab. The idea is to mix the > condition that you currently have (the "base") with all the crystal screen > reagents you have in stock (CS, Index, etc.). As a first trial, use > reservoirs containing 3 parts base and 1 part screen. This generates a new > matrix of hits. You might find a condition that produces thicker crystals > or perhaps a new new crystal form. I think this approach is described in > the literature but don't remember the citation. > > Jack > > > > On Apr 24, 2015, at 12:31 AM, David Briggs wrote: > > Hi, > > In my experience, additive screens (e.g Hampton's) can change crystal > morphology. You could also re-screen for new conditions either using matrix > micro seeding, or change the protein buffer. Perhaps adding a ligand or a > component from your current crystallisation conditions to your protein > stock? > > HTH, > > Dave > > On Fri, 24 Apr 2015 04:02 Prerana G. <[email protected]> wrote: > >> Dear all, >> >> I am working on a protein (40kDa) which forms very thin plate shaped >> crystals which diffracts at very low resolution. Protein concentration that >> i have used for crystallisation is approx. 8mg/ml. I have attached the >> picture of the protein crystal. >> >> >> How can I improve upon the shape of the crystal? >> > > > > John J. Tanner, PhD > Professor of Biochemistry and Director of Graduate Admissions and > Recruitment > Professor of Chemistry (Joint Appointment) > University of Missouri-Columbia > 125 Chemistry Building > Columbia, MO 65211 > email: [email protected] > phone: 573-884-1280 > fax: 573-882-2754 > http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html > > > >
