So far almost everyone is suggesting BME.
I didn't do the purification & crystallization myself, but I am told:
Buffer was HEPES
cryo was ethylene glycol, glycerol and DMSO.
Some MgSO4 and KPO4
No BME, but maybe some DTT.
Purified from a proteobacterium.
On 05/12/15 15:20, Dyda wrote:
What is in buffer? Perhaps DTT and cacodylate?
Fred
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David J. Schuller
modern man in a post-modern world
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