So far almost everyone is suggesting BME.
I didn't do the purification & crystallization myself, but I am told:
Buffer was HEPES
cryo was ethylene glycol, glycerol and DMSO.
Some MgSO4 and KPO4
No BME, but maybe some DTT.
Purified from a proteobacterium.
On 05/12/15 15:20, Dyda wrote:
What is in buffer? Perhaps DTT and cacodylate?
Fred
[32m*******************************************************************************
Fred Dyda, Ph.D. Phone:301-402-4496
Laboratory of Molecular Biology Fax: 301-496-0201
DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov
Bldg. 5. Room 303
Bethesda, MD 20892-0560 URGENT message e-mail: 2022476...@mms.att.net
Google maps coords: 39.000597, -77.102102
http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred
*******************************************************************************[m
--
=======================================================================
All Things Serve the Beam
=======================================================================
David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
