Dear All,
After CCP4 or phenix refinement, I find the H position of N in specific His
residues needs to be regularized based on the function of that His.
Will you please advise on how to actively assign H to one or two of the 2 N in
the His residue in the PDB file? Or do we have a server, which can help us to
assign H to the N of the His in the PDB based on the function of the His, or
based on our intention which N we need to have H in the His?
In addition, after H regularuzed in the His in the PDB, do you think whether
further CCP4 or phenix refinement is needed?
Smith
