Hello everyone I am working on a 5'-nucleotidase (Mg as a cofactor) and have a decent structure of the same at 2A resolution..While doing its biochemical characterization i found it hydrolyzing TMP: Thymine-5'-monophosphate at much higher rate than CMP Cytosine-5'-monophosphate (40 fold difference). Since the structure of TMP and CMP are very similar what could be the possible explanation for this difference or how one should approach to explain this difference..Can modelling, docking and energy minimization give an insight into this direction, if yes then what parameters should be looked into..To summarize..Theoretically how it is possible to explain this difference..??
Thanks in advance -- Regards Faisal School of Life Sciences JNU
