Dear Neeraj,
Do you mean the H3C protease ( trade name: PreScission protease provided by
GE)?
This protease can specifically cleavage the sequence encode by plasmid pGEX-6p,
which possess the GST tag.
Best wishes!
Lu Zuokun
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卢作焜
南开大学新生物站A202
Lu Zuokun, Ph.D. Candidate
College of Life Science, Nankai University
在 2015-06-25 11:09:33，"Neeraj Kr. Mishra" <[email protected]> 写道：
Hello BBians,
sorry for the off-topic question. I have plasmid which possess the precision
protease site to get rid of GST tag. Does anybody have precision protease
plasmid. If someone has please share with me. I will be very thankful for you.
Neeraj
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Neeraj Kr. Mishra
Department of Chemistry
University of Minnesota
Minneapolis, MN 55455
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