Hi Almudena, I'll answer you on the Phenix BB as you've asked specific questions about Phenix tools.
However in general it is a good idea to have a look at your map after phasing and then the (usually two) maps after density modification. If they look like a macromolecule then any of the standard model-building programs should work. All the best, Tom T ________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Almudena Ponce Salvatierra [[email protected]] Sent: Friday, June 26, 2015 4:32 AM To: [email protected] Subject: [ccp4bb] anomalous phasing with HySS - phaser - Autobuild Hi all, I have SeMet data for which HySs locates 127 Selenium atoms with a CC of 0.31, which I think is decent. Then I run Phaser to generate the first maps, and it gives a score of 25 and LLG of 190 or so... The next step would be running Autobuild, however, the first models I realize it is building have R work and R free between 50 and 55%. So I guess it is just some random stuff there. If the result from HySS and Phaser looks more or less good, why does AutoBuild show these numbers?? Any idea of what I could do to improve so? Any ideas are welcome. Best wishes, Almudena -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
