1) Commercially made oligonucleotides 2) M13 rolling circle replication (how people used to make ssDNA for sanger sequencing).
——————————————————— Dr Antony W Oliver Senior Research Fellow CR-UK DNA Repair Enzymes Group Genome Damage and Stability Centre Science Park Road University of Sussex Falmer, Brighton, BN1 9RQ e: [email protected]<mailto:[email protected]> t(office): +44 (0)1273 678349 t(lab): +44 (0)1273 677512 http://www.sussex.ac.uk/lifesci/oliverlab http://tinyurl.com/aw-oliver [cid:[email protected]] On 2 Jul 2015, at 16:40, Reza Khayat <[email protected]<mailto:[email protected]>> wrote: Hi, Sorry for the non-crystallography question, does anyone know how to produce milligram quantities of single stranded DNA? Thanks. Best wishes, Reza Reza Khayat, PhD Assistant Professor Department of Chemistry City College of New York 85 Saint Nicholas Terrace, CDI 12318 New York, NY 10031 http://www.khayatlab.org/ 212-650-6070
