Dear Dilip, I was wondering if the problem was more basic, just about setting the occupancy in your input pdb file. Try setting the occupancy of Mg to 1 in your input coordinate file (pdb file) and refine it. If its Mg, it should turn blue. If its not Mg, the density should turn red instead of staying green, or may be partially green. Next, try Mn with occupancy 1 in the input file. Did you have Calcium chloride in your crystallization condition? You could try Ca as well.
-Gyan On Thu, Jul 9, 2015 at 4:35 AM, Dilip Kumar <[email protected]> wrote: > Dear All > > I have solved a structure of a metal-ion dependent exonuclease enzyme. In > homologous structures, two or three Manganese ions are present at catalytic > center. However, I have used 2 mM MgCl2 in protein purification buffer. I > tried to fit both of these metal ions at catalytic center but in both cases > it still shows green density (Sigma level ~ 7) in difference map and low > b-factor (<10) for these metal ions. For better understanding I have > attached the screenshot of metal ions with difference map on. Please > suggest me the possible reasons or methods to validate the presence of any > other metal ions at catalytic center. > > Thanks in advance. > > Regards > Dilip Kumar > Research Associate > Chemical and Systems Biology Unit > CSIR-Institute of Genomics & Integrative Biology > Delhi-110025 > -- Gyanendra Kumar, PhD St. Jude Children's Research Hospital, Department of Structural Biology, 262, Danny Thomas Place, MS-311 Memphis, TN 38105 Phone: 901-595-3839 Cell: 631-875-9189 -------------------------------------------------------
