Diamond and a variety of other SRs have these devices. Check out their webpages for available equipment or contact directly.
Dave -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Phil Evans Sent: 05 October 2015 09:59 To: ccp4bb Subject: Re: [ccp4bb] Advice for Structure Determination Xe at longish wavelength (> 1.5Å), if you can find a pressure device (LURE maybe?) Phil > On 5 Oct 2015, at 09:08, Marc Graille <[email protected]> wrote: > > Dear Monica, > > I had solved couple of structures using anomalous signal for tungsten. I have > incubated the proteins with 25-50mM NaWO3 and set-up the crystallization > trays. > If you are lucky, WO3 might interact with your protein as SO4 ions do > sometimes and you might be able to get anomalous signals. > This works fine for nucleic acid binding proteins. > > Otherwise, I have also solved a structure of a protein expressed in Pichia > using anomalous signal from SeMet. It might be time-consuming and complicated > to get labeled protein in Pichia but could be faster than heavy atoms > derivatives. > Here is a link to the publication describing the SeMet labeling procedure. > http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/26082394 > > Hope it helps, > > Good luck, > > Marc > >> Le 5 oct. 2015 à 09:38, Monica Mittal <[email protected]> a écrit : >> >> Dear all, >> >> I need a general advice. I don't have a suitable Model (Lets say only with a >> similarity of 15%) and no other model to solve the structure by MR. Then i >> think of anomalous experiments, but soaking with heavy metals is not good >> for the crystals. Since the protein is being expressed in Pichia cells, >> growth in Seleno-media is cumbersome. There are no ligands or ions that i >> can use for anomalous scattering of protein crystals. I am trying to express >> it in bacteria but have some issues. What is the option that i can choose to >> solve the structure of such a protein ? Your suggestions are highly >> recommended. >> >> Thanks in advance. >> Monica > > Marc GRAILLE, PhD > Directeur de recherche CNRS > > Laboratoire de Biochimie > ECOLE POLYTECHNIQUE - UMR7654 CNRS > 91128 PALAISEAU CEDEX > > <Signature-email.jp2> > > Phone : +33 (0)1 69 33 48 90 – Fax : +33 (0)1 69 33 49 09 > > Email: [email protected] > > Team: Translation and degradation of eukaryotic mRNAs > http://bioc.polytechnique.fr/spip.php?rubrique117 > Team supported by the ATIP-Avenir CNRS program > > > -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
