Colleagues, Working at relatively high resolution of 1.5A allows in some cases seeing alternative positions of side chains. After placing those in the coordinate file with occ adding to 1.0 and running refinement (latest REFMAC on Win) I find that occupancies of all atoms in the specific residue with altlocs are being modified. In the refinement through GUI I've failed to find place where occ refinement is turned on and assumed that occ refinement will be automatically turned on upon presence of altlocs. Apart from being aware of danger associated with some assumptions would you be able to advise what I'm missing in refinement with altlocs? Thank you in advance.
Regards, Vaheh Oganesyan MedImmune, ADPE www.medimmune.com To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
