Microbatch under oil in Terasaki plates or perhaps microdialysis might be an 
option.
Then you don’t have a liquid/air interface. It is harder to do very small 
volumes then, especially with microdialysis.
Or free interface diffusion in thin tubes (or with specialised equipment).

Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
calle Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://wwwuser.cnb.csic.es/~mjvanraaij







> On 17 Nov 2016, at 15:14, [email protected] <[email protected]> wrote:
> 
> Dear All,
>  
> How to avoid the skin on the drop? These skins are believed to be a layer of 
> denatured protein or caused by the polyethylene glycol. 
> They will certainly slow down the rate of vapor diffusion. If you have any 
> good suggestions to avoid skin forming? 
>  
> Best wishes,
> Xiaorong Li
> 
> [email protected]

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