Hi All,
I am using CCP4i 7.0.0.025 linux version. I am wondering if Refmac5 automatically does density modification (e.g. solvent flattening, NCS, etc..) in the refinement after I put a solution coordinate from Phaser MR molecular replacement and the experiment data (MTZ file)? If Refmac5 does not do density modification automatically, is it better that first doing a density modification (CCP4i DM or Pirate) after molecular replacement (input the solution coordinate file and mtz map generated by Phaser MR), and then building structures and finally do the Refmac5?
