Re: [ccp4bb] SeMet in S2 cell

[Engin Özkan]

Nothing specific about S2 cells to contribute... However, S2s usually 
grow fine in other insect media, such as those intended for lepidopteran 
cell lines like the Sf9 and High Five. Protocols established for those 
cell lines, for example, check out those using the medium ESF-921, would 
probably work for you. Please let us know what works out.

Engin

On 12/21/16 6:15 PM, Xianchi Dong wrote:
Dear All,

Does anybody have experience with SetMet protein in S2 cells? What 
kind of reagent should I use and is anything else I should pay 
attention to?
Thanks in advance.

Best,
Xianchi