Dear all,
We are trying to solve a protein structure of 37KDa using molecular replacement method. Protein secondary structure indicates that it has 4-Heat repeat (α-helical hair pin) i.e. 16 α-helices. It has about 40% sequence identity with templates in PDB, using which we tried MR, but we are unable to find a solution using MOLREP, PHASER, BALBES, MRBUMP, MORDA etc. This protein has two iron molecules also and data were collected at home source using Cu Ka radiation with 1.5418 A wavelength at 2.5 A resolution. Its highest homology PDB template is also having 16 α-helices with horseshoe shaped structure. We are also trying to use Arcimboldo tool (in the CCP4 7.0) to find the structure solution, but it is taking a long time on our computers (8-core). So I will be grateful if anyone can provide us suggestions 1. Any program which can provide us good working model having only α-helical content. 2. In case we know our model, how can we optimize Arcimboldo tool with our helical model (we can generate from homologous PDB) Thanking you in advance, Madhusudhan
