Hi Tomas, Thanks. I think this one is directed at a C-terminal his6-tag. I'm not sure it'll work with an N-terminal tag.
Elias ________________________________ From: Tomas Malinauskas <tomas.malinaus...@gmail.com> Sent: Sunday, January 8, 2017 5:39:59 PM To: Fernandez, Elias J Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] anti-hexahistidine antibody Dear Fernandez et al., maybe this anti-His-tag antibody could be useful: PDB ID 1KTR, its sequence is in the paper https://www.ncbi.nlm.nih.gov/pubmed/?term=12054774 Hope that helps, Tomas On Sun, Jan 8, 2017 at 9:24 PM, Fernandez, Elias J <efern...@utk.edu> wrote: > Dear All, > > Is anyone aware of a recombinant system that produces an N-terminal, > hexa-histidine-directed Fv antibody fragment that can be used for structural > studies (crystallography, SAXS, cryo-EM)? > > Best regards, > > Elias > > >
