Dear all,



we are seeking an enthusiastic post-doctoral researcher to join the Klaholz 
group at the Centre for Integrative Biology, IGBMC, Illkirch, France 
http://igbmc.fr/Klaholz



We are studying the structure and function of protein complexes through 
integrated structural biology and notably by high resolution cryo-EM. We are 
looking for a highly enthusiastic and motivated scientist with relevant 
experience in structural biology of DNA/RNA-protein complexes, ideally with 
good experience in cryo electron microscopy. For further details please see 
below.



With best regards,



Bruno Klaholz









Some recent publications:

-  I. Orlov, A. G. Myasnikov, L. Andronov, S. K. Natchiar, H. Khatter, B. 
Beinsteiner, J-F. Ménétret, I. Hazemann, K. Mohideen, K. Tazibt, R. Tabaroni, 
H. Kratzat, N. Djabeur, T. Bruxelles, F. Raivoniaina, L. di Pompeo, M. Torchy, 
I. Billas, A. Urzhumtsev & B. P. Klaholz. The integrative role of cryo electron 
microscopy in molecular and cellular structural biology. Biol Cell., 2016, Oct 
12. doi: 10.1111/boc.201600042.

-  A. G. Myasnikov, S. K. Natchiar, M. Nebout, I. Hazemann, V. Imbert, H. 
Khatter, J.-F. Peyron & B. P. Klaholz. Structure-function insights reveal the 
human ribosome as a cancer target for antibiotics. Nat Commun., 2016, 7, 12856. 
doi: 10.1038/ncomms12856.

-  A. Simonetti, J.-F. Ménétret, F. Martin, A. G. Myasnikov, Q. Vicens, L. 
Prongidi-Fix, S. K. Natchiar, B. P. Klaholz & G. Eriani. Ribosomal 18S rRNA 
base pairs with mRNA during eukaryotic translation initiation. Nat Commun., 
2016, 7, 12622. doi: 10.1038/ncomms12622.

-  L. Andronov, I. Orlov, Y. Lutz, J-L. Vonesch & B. P. Klaholz. ClusterViSu, a 
method for clustering of protein complexes by Voronoi tessellation in 
super-resolution microscopy. Sci. Rep. 2016, 6, 24084. doi: 10.1038/srep24084.

-  L. Andronov, Y. Lutz, J-L. Vonesch & B. P. Klaholz. SharpViSu: integrated 
analysis and segmentation of super-resolution microscopy data. Bioinformatics, 
2016, 32, 2239-2241. doi: 10.1093/bioinformatics/btw123.

-  B. P. Klaholz. Structure sorting of multiple macromolecular states in 
heterogeneous cryo-EM samples by 3D multivariate statistical analysis. Open 
Journal of Statistics, Special Issue on Multivariate Statistical Analysis, 
2015, 5, 820-836. http://dx.doi.org/10.4236/ojs.2015.57081.

-  B. Beinsteiner, J. Michalon & B. P. Klaholz. IBiSS, a versatile and 
interactive tool for integrated sequence and 3D structure analysis of large 
macromolecular complexes. Bioinformatics, 2015, 31, 3339-3344. doi: 
10.1093/bioinformatics/btv347.

-  H. Khatter, A. G. Myasnikov, K. Natchiar & B. P. Klaholz. Structure of the 
human 80S ribosome. Nature, 2015, 520, 640-5. doi: 10.1038/nature14427. Epub 
2015 Apr 22.

-  Z. A. Afonina, A. G. Myasnikov, V. A. Shirokov, B. P. Klaholz, A. S. Spirin. 
Conformation transitions of eukaryotic polyribosomes during multi-round 
translation. Nucleic Acids Res. 2015, 43, 618-28. doi: 10.1093/nar/gku1270.

-  A. G. Myasnikov, Z. A. Afonina, J-F. Ménétret, V. A. Shirokov, A. S. Spirin 
& B. P. Klaholz. The molecular structure of the left-handed supra-molecular 
helix of eukaryotic polyribosomes. Nat Commun., 2014, 5, 5294. doi: 
10.1038/ncomms6294.

-  M. Maletta, I. Orlov, P. Roblin, Y. Beck, D. Moras, I. M. L. Billas & B. P. 
Klaholz. The palindromic DNA-bound USP/EcR nuclear receptor adopts an 
asymmetric organization with allosteric domain positioning. Nat Commun., 2014, 
5, 4139. doi: 10.1038/ncomms5139.



For ongoing projects and full publication list of the team see 
http://igbmc.fr/Klaholz



Our group is located at the Centre for Integrative Biology (CBI) at IGBMC, 
Illkirch/Strasbourg, France, which comprises cutting-edge cryo-EM facilities:

The CBI provides a leading-edge scientific and technological environment in 
integrated structural biology to address the structure and function of 
biological systems, notably on gene expression, from the atomic, molecular to 
the tissue scales. The CBI http://www.igbmc.fr/grandesstructures/cbi/, hosts 
the French and European Infrastructures for Integrated Structural Biology, 
FRISBI http://frisbi.eu/  and Instruct https://www.structuralbiology.eu/  which 
comprises advanced electron microscopy facilities equipped with cutting-edge 
instrumentation such as Titan Krios and Polara cryo electron microscopes, cryo 
Focused Ion Beam Scanning Electron Microscope (cryo-FIB/SEM) and 
super-resolution fluorescence microscopy. The Titan Krios microscope is 
equipped with Cs corrector, Falcon 2 camera, GIF energy filter, K2 camera and 
phase plate. In addition, the EM facility has a suite of associated equipments 
for sample preparation and dedicated computing resources for image processing 
and 3D reconstruction by single particle cryo-EM and cryo electron tomography 
(cryo-ET).



Applications should be sent via email to 
[email protected]<mailto:[email protected]> including CV, list of publications, 
names of 3 referees and motivation letter. The position remains open until 
filled.







###########################################################################
Bruno P. Klaholz
Centre for Integrative Biology
Department of Integrated Structural Biology
Institute of Genetics and of Molecular and Cellular Biology
IGBMC - UMR 7104 - U 964
1, rue Laurent Fries
BP 10142
67404 ILLKIRCH CEDEX
FRANCE
Tel. from abroad: 0033.369.48.52.78
Tel. inside France: 03.69.48.52.78
websites:
http://igbmc.fr/Klaholz
http://www.igbmc.fr/grandesstructures/cbi/
http://frisbi.eu




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