Yes if it either A) oligomerizes B) significantly changes shape C) aggregates reversibly
Artem www.harkerbio.com "where wild SEC columns roam free" On Jan 11, 2017 7:22 PM, "Reza Khayat" <rkha...@ccny.cuny.edu> wrote: > Hi, > > > Sorry for the off-topic question. Can a protein in lower [NaC] run faster > on a SEC than at higher [NaCl] (i.e. elute at an earlier volume)? The > protein elutes well within the resolution limits of the SEC > with a symmetric gaussian A280 profile. I know that at lower [NaCl] the > protein can elute later because it may interact with the matrix. Thanks. > > > Best wishes, > Reza > > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031 > > >
