Following on I read somewhere a while back that potassium conc in E. coli is estimated in the 30-300 mM range (http://book.bionumbers.org/what-are-the-concentrations-of-different-ions-in-cells/ <http://book.bionumbers.org/what-are-the-concentrations-of-different-ions-in-cells/>) . In other more extremophiles it can be higher (Extremophiles <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339784/#>. 2015; 19(2): 315–325.) Maybe our default buffers should contain K+ and Glu at such high conc- though not compatible for IEX of course it would appear that such conditions are physiological at least for intracellular bacterial proteins.
Prof. Jon R Sayers FRSB Dept. of Infection, Immunity & Cardiovascular Disease University of Sheffield Medical School Beech Hill Rd Sheffield S10 2RX United Kingdom, Tel +44 (0)114 215 9552 Fax +44 (0) 114 271 3892 Email [email protected] http://www.sheffield.ac.uk/iicd/profiles/sayers <http://www.sheffield.ac.uk/iicd/profiles/sayers> > On 12 Jan 2017, at 09:13, Reza Khayat <[email protected]> wrote: > > I don't think this is taught in Biochem101. You didn't miss it. The cytoplasm > is quite viscous, like jello. > > > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031 > > ________________________________________ > From: CCP4 bulletin board [[email protected]] on behalf of Tim Gruene > [[email protected]] > Sent: Thursday, January 12, 2017 3:55 AM > To: [email protected] > Subject: Re: [ccp4bb] Completely Off-Topic > > Dear JPK, > > I was not aware of the absolute numbers, but maybe they are little suprising: > when your tinned food contains 'yeast extract' it is equivalent to monosodium > glutamate, which is commonly used as flavour enhancing agent. > > I am not a chemist to worry about it, but yeast seems to have a fullfilling > life with it. > > Best, > Tim > > On Thursday, January 12, 2017 12:45:03 AM CET Keller, Jacob wrote: >> Dear Crystallographers, >> >> Was anyone else aware that in E coli the intracellular glutamate >> concentration is ~100 mM? Also other cell types (yeast, mammalian) are 10s >> mM. Anything to say about this? I learned of this just recently, and have >> been amazed about it for more than a week. Did I miss this in Biochem 101? >> Does it matter? >> >> JPK >> >> ******************************************* >> Jacob Pearson Keller, PhD >> Research Scientist >> HHMI Janelia Research Campus / Looger lab >> Phone: (571)209-4000 x3159 >> Email: [email protected]<mailto:[email protected]> >> ******************************************* > > -- > -- > Paul Scherrer Institut > Tim Gruene > - persoenlich - > OFLC/102 > CH-5232 Villigen PSI > phone: +41 (0)56 310 5297 > > GPG Key ID = A46BEE1A Best wishes, Prof. Jon R Sayers FRSB Dept. of Infection, Immunity & Cardiovascular Disease University of Sheffield Medical School Beech Hill Rd Sheffield S10 2RX United Kingdom, Tel +44 (0)114 215 9552 Fax +44 (0) 114 271 3892 Email [email protected] http://www.sheffield.ac.uk/iicd/profiles/sayers
