Thanks. I did the following for each operator and worked fine. The CHAIN keyword renamed the original chain names. Armando
# pdbset xyzin 5k7l.pdb xyzout 5k7l_GH.pdb << eof-1 transform 0.000000 1.000000 0.000000 -1.000000 0.000000 0.000000 0.000000 0.000000 1.000000 0. 332.79999 0 CHAIN A G CHAIN B H end eof-1 El 18/01/2017, a las 11:16, Tim Gruene escribió: > Dear Armando, > > did you try the TRANSFORM command in pdbset? It seems appropricate to > generate > the other molecules. You probable need to do it separately for each operator > (except the first one, of course, the identity). > > Best, > Tim > > On Wednesday 18 January 2017 11:13:21 AM Armando Albert wrote: >> Dear all, >> Does any one know how to generate a complete biomolecule out of the >> BIOMOLECULE record from a pdb file that contains one of the four molecules >> forming a tetramer This is an Crio-EM model. >> Armando >> >> >> >> REMARK 350 BIOMOLECULE: 1 >> REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B >> REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 >> REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 >> REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 >> REMARK 350 BIOMT1 2 0.000000 -1.000000 0.000000 332.79999 >> REMARK 350 BIOMT2 2 1.000000 0.000000 0.000000 0.00000 >> REMARK 350 BIOMT3 2 0.000000 0.000000 1.000000 0.00000 >> REMARK 350 BIOMT1 3 -1.000000 0.000000 0.000000 332.80000 >> REMARK 350 BIOMT2 3 0.000000 -1.000000 0.000000 332.79999 >> REMARK 350 BIOMT3 3 0.000000 0.000000 1.000000 0.00000 >> REMARK 350 BIOMT1 4 0.000000 1.000000 0.000000 0.00000 >> REMARK 350 BIOMT2 4 -1.000000 0.000000 0.000000 332.79999 >> REMARK 350 BIOMT3 4 0.000000 0.000000 1.000000 0.00000 >> REMARK 465 > -- > -- > Paul Scherrer Institut > Dr. Tim Gruene > - persoenlich - > Principal Investigator > Biology and Chemistry > OFLC/102 > CH-5232 Villigen PSI > > Phone: +41 (0)56 310 5297 > > GPG Key ID = A46BEE1A >
