Try Tricine gel. It is particularly suited for low molecular wt proteins and it will give you very good resolution. Even you can run it overnight at 30 V (16-18 hours).
On Thu, Jan 19, 2017 at 9:33 AM, Walt <ofe...@gmail.com> wrote: > Hi, > > I have a small protein (~9 kDa) with acidic pI (~4). > When I run 18% native-PAGE, it appears my protein is in the dye front. > How can I fix this problem? Changing the pH of separating gel > might help? How about gradient native-PAGE? Thank you! > > Walt >
