Hi Patrick:
We have used the Clonetech Chaperone Coexpression Kit. That includes
GroEL/GroES plasmids.
http://www.clontech.com/BT/Products/Protein_Expression_and_Purification/Bacterial_Expression_Systems/Protein_Folding/Chaperone_Plasmid_Set
Hope this helps,
Alexandra
On 1/19/17 9:19 PM, Patrick Loll wrote:
Hello everyone,
Please allow me to kill two off-topic birds with one email ‘stone’:
1) Can anyone suggest where I can lay hands on the pREP4-GroESL
plasmid for bacterial co-expression of GroEL and GroES? I’ve checked
with the original author (Philip Cole), but it’s been decades since he
published on this, and he’s not sure he can find the plasmid. However,
I see recurrent (and recent) references to the plasmid in the
literature, so I guess that it’s loose in the wild…
2) We’re looking for a contract research organization to do some
Bac-Mam expression for us (we’re in a hurry to get some results, so
we’d like to farm it out rather than get the technology going
in-house). Can anyone recommend a group (ideally in the US, for
logistical ease)?
Thanks for any help.
Cheers,
Pat
---------------------------------------------------------------------------------------
Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA19102-1192USA
(215) 762-7706
pjl...@gmail.com <mailto:pjl...@gmail.com>
pl...@drexelmed.edu <mailto:pl...@drexelmed.edu>
--
Alexandra M. Deaconescu, B.E., Ph.D.
Assistant Professor of Molecular Biology, Cell Biology and Biochemistry
