I am trying to refine my data set collected at 1.9A. The density appears clean and the fit is also good. In coot, all residues are in green zone in density fit analysis. Also in Ramachandran plot 89% residues are in allowed region. Few residues in loop region do not have good density for side chains. My protein contains about 61% loops and remaining beta sheets , no alpha helices (predicted from homology model). What could be done to improve the Rfree and Rfactor?
- [ccp4bb] Rfactor and Rfree not coming below 0.4 ameya benz
- Re: [ccp4bb] Rfactor and Rfree not coming below 0.4 Dale Tronrud
- Re: [ccp4bb] Rfactor and Rfree not coming below 0.... Eleanor Dodson
- Re: [ccp4bb] Rfactor and Rfree not coming below 0.4 Bernhard Rupp
