The truth is in the map. Ergo, zap it and rationalize why it worked or not later.
Cheers, BR From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Markus Heckmann Sent: Tuesday, January 24, 2017 6:23 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] add ligand solution onto drop directly Dear all, I wondered if it is OK to pipette ligand soln (X-CoA) *directly* to the drop with crystal (1:1 ratio of protein:precipitant 2µl) instead of dissolving it in precipitant solution and transferring the crystal to this ligand containing precipitant solution. The crystals survive this as I add the ligand solution to the edge of the drop and gently mix the two solutions. Since I collected my datasets I wonder if it is OK? Many thanks, Markus
