You mentioned that you have PMSF in your crystallization condition…and PMSF does modify serines.
Best regards, Z *********************************************** Zachary A. Wood, Ph.D. Associate Professor Department of Biochemistry & Molecular Biology University of Georgia Life Sciences Building, Rm A426B 120 Green Street Athens, GA 30602-7229 Office: 706-583-0304 Lab: 706-583-0303 FAX: 706-542-1738 *********************************************** > On Jan 26, 2017, at 11:13 AM, Mark J van Raaij <[email protected]> wrote: > > well yes, looks like a glycosylation to me too. > However, at 2.75Å resolution I think it will be impossible to know which one > from the density alone. > Perhaps mass spectroscopy can give a clue? > Or get clues from what is known about your protein and your expression system. > > Mark J van Raaij > Dpto de Estructura de Macromoleculas > Centro Nacional de Biotecnologia - CSIC > calle Darwin 3 > E-28049 Madrid, Spain > tel. (+34) 91 585 4616 > http://wwwuser.cnb.csic.es/~mjvanraaij > > > > > > > >> On 26 Jan 2017, at 15:45, KH. HEROJIT SINGH, PH D SCHOLAR, PCL >> <[email protected]> wrote: >> >> Dear All, >> >> >> A continuous map from gamma O of serine at expose region was observed. >> Resolution of dataset is 2.75 A. The serine position is predicted for >> phosphorylation in-sillico. With fitting with mannose, density is not fully >> satisfied while with GalNac, some negative density is found. >> >> >> Condition was 15% PEG 3350, Tris 50mM pH 7, 100mM Na2SO4and buffer was tris >> 50mM, NaCl 50mM, ph 8.5 , 1mM PMSF. Images from different angle are attached >> (File size is 300Kb). Arrow in fig pointed the same serine residue. >> >> >> Kindly suggest. >> >> -- >> Regards, >> KHUNDRAKPAM HEROJIT >> Ph D SCHOLAR >> PROTEIN CRYSTALLOGRAPHY LAB. >> NATIONAL INSTITUTE OF IMMUNOLOGY >> NEW DELHI-110067, INDIA<ccp4.jpg>
