I always use DSSP, which I believe has been the gold standard for secondary structure assignment from pdb files for many years. PYMOL also has a DSSP plugin that can be used to override its own secondary structure assignment, which is nowhere near as good as DSSP.
Best Tony ------------------------------------------------------ Dr. Antonio Ariza University of Oxford Sir William Dunn School of Pathology South Parks Road Oxford OX1 3RE Links to my public profiles: ResearchGate<https://www.researchgate.net/profile/Antonio_Ariza> LinkedIn<https://www.linkedin.com/in/antonioariza1> GoogleScholar<https://scholar.google.co.uk/citations?user=9pAIKV0AAAAJ&hl=en> ORCID<https://orcid.org/0000-0003-4364-823X> ________________________________ From: CCP4 bulletin board [[email protected]] on behalf of chemocev marker [[email protected]] Sent: 29 January 2017 10:41 To: [email protected] Subject: [ccp4bb] secondary structure assignment to PDB file Hi Is there any tool that can assign secondary structure to the PDB file. The problem is if I used different modelling tools, there are regions in the protein which does not remain consistent and looks different in different application. best J. Vitali
