Does this count as an example? grep SSBOND /a/pdb/pdb4e9m.ent SSBOND 1 CYS A 39 CYS B 39 1555 1555 2.05 SSBOND 2 CYS C 39 CYS D 39 1555 1555 2.04 SSBOND 3 CYS E 39 CYS F 39 1555 1555 2.03
The A.U. contains three domain-swapped dimers. The cys are not on the swapped helix but the swapping fortuitously brings the same cys in the two molecules into proximity to make a disulfide. There are two or three other x-ray structures that show the same domain-swapped, disulfide-clinched dimer in different packing. However an NMR structure shows it to be monomeric in solution, based on estimated tumbling speed since nmr restraints might not distinguish inter- from intramolecular contacts in a domain-swapped dimer. The cys is not conserved, and although this protein is expected to oligomerize, the putative oligomerization domain is not included in this construct. On 02/01/2017 10:17 AM, Eleanor Dodson wrote:
Does anyone know of examples of these? I have found one - 2WQW with these SSBOND records 2WQW SSBOND 1 CYS A 206 CYS A 227 1555 6556 2.07 SSBOND 2 CYS B 206 CYS B 227 1555 5556 2.15 We seem to have one but it would have to form after crystalisation? Eleanor
