Speaking specifically for succinate dehydrogenase, there are a number of
assembly factors required for insertion of flavin, iron-sulfur clusters
(SDHAF1,2,3 . . .). Since E. coli or Pischia make their own SDH, there is
a possibility the endogenous assembly factors and co-factors would work
with the heterogenous proteins. Not a sure thing though, so don't ask NIH
to fund it until after you've got it working!
eab
On 02/03/2017 03:46 AM, Fulvio Saccoccia, Sapienza wrote:
Dear ccp4ers,
I was wondering about most recent advances in production and
crystallization of complexes of multisubunit membrane proteins, for
instance succinate dehydrogenase, pyruvate dehydrogenase complex or even
photosystem complex. As far as I know, many large complexes of membrane
proteins were produced by tittues extraction rather than by recombinant
expression but I want to know if there is room to work with these large
assemblies by using recombinant proteins.
Best wishes
Fulvio
