Hard to see but maybe a transesterified serine like ser-o-AMP Can you share the nature of the enzyme?
Artem www.harkerbio.com "Zoidberg was here" On Feb 4, 2017 10:14 AM, "sharifah nur hidayah syed mazlan" < shn.hida...@gmail.com> wrote: > Dear All, > > I am working on a structure with an unknown blob extended from the gamma O > of the catalytic serine residue. The resolution of the dataset is 1.38 A. I > have no idea whether the residue is modified or the blob belongs to other > molecule. > > The protein was expressed in Rosettagami (DE3), purified using > Ni-Sepharose (affinity chromatography) and Q-Sepharose (Anion exchange > chromatography). The crystallization formulation used contain 15% PEG 8000, > 0.2 M Ammonium sulphate, 0.1 M sodium cacodylate trihydrate pH 6.5; and the > buffer composed of 50 mM Sodium chloride and Phosphate buffer pH 8. No > protease inhibitor was used (eg: PMSF) > > I have tried to fit in diethylene glycol as shown in one of the attached > figures, but as observed, it is not really fit and the molecule is in > incorrect conformation. > > Kindly help me with this matter. > > > > Thanks and regards, > > Sharifah Nur Hidayah > Universiti Putra Malaysia, > Malaysia > > >
