Hello All I am using CM Sephadex C50 column for my protein. I equilibrated column with MOPS buffer pH 6.0. But during elution I am using only NaCl2. This looks like changing the bed height. Before starting elution the bed height was around 60cm. After starting elution the bed height decreased and came upto 12 cm only. After 1M NaCl elution I cleaned the column with ddH2O. The column begin to enlarge to the height of more than 60cm. I dont understand this behaviour.
More over I could not find my protein as well. Similar situation arised even if I change the buffer (Acetate buffer). Any suggestions? Thank you Syed
