We have had good luck with columns from Essential Life Solutions ( http://www.essential-life.net/). They are easy to work with and hold up under pressure well.
Best, David -- David L. Blum, Ph.D. Director, Bioexpression and Fermentation Facility Department of Biochemistry and Molecular Biology University of Georgia 120 Green Street room A414A Athens, GA 30602 http://bff.uga.edu/ Skype: dlblum11 (706) 542-1035 (Office) (706) 542-1077 (Fax) On Wed, Feb 15, 2017 at 1:04 PM, Carlos Kikuti <[email protected]> wrote: > Well GE sells empty columns (XK for low back-pressure, tricorn for higher > back-pressure) that you can fill with the resin you want. They seem > expensive but for that kind of things I don't think any option will be real > 'savings'. For nickel affinity we have been having good results with > Roche's Complete - it requires less imidazole for binding and elution, and > at the same time it is compatible with reducing agents and chelators. And > we still buy the HisTrap FF crude. > > On Wed, Feb 15, 2017 at 6:03 PM, Christian Roth < > [email protected]> wrote: > >> Hi Markus, >> >> BioRad has columns as well (prepacked and empty), which follow the >> establiched connector standard. So usable without the need of adapters. >> >> Cheers >> >> Christian >> >> Am 15.02.2017 um 16:57 schrieb Markus Seeliger: >> >> Dear all, >> we are happy users of all that GE offers around their FPLC system, but I >> am getting a little tired of feeling monopolized. Are any of you aware of >> either empty columns or prepacked columns (e.g. metal affinity or ion >> exchange resins) from other companies? >> Thanks for your advice >> >> Markus >> >> >> >
