Dear CCP4bb members, My apologies for posting non crystallography question. This relates to protein aggregation thus I thought of asking to the board members experienced in this.
I am working on an Antibody-small molecule conjugation which involves NHS mediated coupling. Upon conjugation I've attempted to purify the conjugate using cation exchange followed by SEC. In SEC, everything seems to elute in void volume and interestingly I also note remaining free Ab eluting at a volume appropriate for its size. In our downstream tests, we see lots of noise typical of aggregates (protein or non-protein). So far we have tried a few additives but not much improvements in noise reduction. I would appreciate your kind advice. Best. Ha
