Dear Debiasish,
more than "quasicrystals" (crystal in which
molecules adopt an ordered but aperiodic structure), to me the one in your
photo looks like spherulites. You shouldn't be far for finding the right
crystallization conditions: maybe change the pH of your precipitant and try
lower concentration of protein or precipitant or both at the same time.
Camillo
On Fri, Mar 24, 2017 at 12:10 AM, Debasish Kumar Ghosh <[email protected]>
wrote:
> Dear All,
>
> I have a small doubt regarding possibility of formation of quasi-crystal
> of protein complex. I am trying to co-crystallize a heterodimeric protein
> complex of two small proteins (14KDa and 16KDa, both are human origin). One
> of them is a membrane protein. we have extensively characterized their
> qualitative and quantitative binding properties (with IP/IB, Confocal
> microscopy, ITC etc.). We are quite certain of their strong 1:1 binding
> stochiometry.
> In some crystallizing conditions, we are getting some structures which
> appears to me as quasi-crystals (Image attached). However, I am not coming
> across ample examples of quasi-crystals of protein complexes.
> I will be very keen to know if anyone had this (similar) kind of
> experience(s). And of course it would be wonderful to know if they are
> diffractable and, if yes, what are the odds of having good diffraction.
>
>
> Best !!
>
> Debasish
>
> CSIR- Senior Research Fellow
> Computational and Functional Genomics Group
> Centre for DNA Fingerprinting and Diagnostics
> Hyderabad, INDIA
>
>
