Hi Steven,
In a word, lots :) In somewhat more detail .... If this is a request to the CCP4 community, then the images and crystallization metadata from the Hauptman-Woodward Medical Research Institute High-Throughput Crystallization Center are packed in a rar format and users of the Center on CCP4bb can provide those directly to you. They are readable with the program MacroscopeJ, available from http://hwi.buffalo.edu/science/high-throughput-crystallization-center/analyzing-your-results/. The software is freely available. The Center itself does not share users images or data. There are many examples of the images given in the publications listed on the crystallization research link to the Center, http://hwi.buffalo.edu/science/high-throughput-crystallization-center/crystallization-research/. The most up to date example is Luft, et al., (2015) Struct Dyn, 2, 041710 which is open access. Images in that paper were recorded by the Crystallization Screening Center and typical of those obtained. Further details and other image examples are given in the references cited within. The Center encourages potential users to check the publications list at the link above to best understand the capabilities, effective use, and optimization strategies. A complete data set involves crystallization metadata and 1,536 images before the sample is added, immediately after, one day after, one week, two weeks, three weeks, and four, five, and six weeks after. These images are color video microscope images (recently upgraded from B/W) and are supplemented with a set of SONICC and UV-TPEF images. I'll ask the Center to add a one of the QC sample sets or one from the many PSI program samples to the website for those that are interested. In each set you'll have almost 17,000 images to play with but at least it will be a good demonstration of the analysis software! I don't believe it's appropriate to use the CCP4 board as an advertising medium and I apologize for this posting that comes close to that. I'd request that questions about the crystallization screening service be directed to the contacts on the getacrystal.org page. I'm sure they'd be happy to answer any specifics and they would be a appropriate direct contacts to get image examples than the bulletin board in general. Best wishes and many successful crystallizations, Eddie. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu<http://hwi.buffalo.edu/> Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: [email protected]<mailto:[email protected]> [https://mail.google.com/mail/u/0/?ui=2&ik=62aec2015e&view=fimg&th=15b6914f7fdf6013&attid=0.1&disp=emb&attbid=ANGjdJ9g06QLo-D_EH41Fh-YwWWc3MjxJbO2-5rUPyw9xaU5whcTtrcEMgp6IRAWj-flG5nAubbpuMSCQvS1Drhckx7xSgFjo3hKVAHILDzuOysZW5m7BjsDwfq3SJc&sz=w656-h112&ats=1492181842461&rm=15b6914f7fdf6013&zw&atsh=1] Heisenberg was probably here! ________________________________ From: Steven Herron <[email protected]> Sent: Saturday, April 15, 2017 6:46 PM To: Edward Snell; [email protected] Subject: Re: [ccp4bb] Recommendations for Robotic Crystal Screening Services Does the Hauptman-Woodward Medical Research Institute have any sample data-sets, so we can see what the results look like? On 4/14/2017 8:58 AM, Edward Snell wrote: Dear Elizabeth, The High-Throughput Crystallization Screening Center and the Hauptman-Woodward Medical Research Institute has been operating for over a decade with considerable success. There are comprehensive details at http://<http:>getacrystal.org but basically your samples are screened against a large range of commercially available conditions plus some more unique ones. Video microscope images are provided over a period of six weeks or longer by request and the imaging also includes SONICC (detecting very tiny crystals or crystals in precipitate) and UV-TPEF to ensure the sample is protein. There are a large range of analysis tools that can be used on the data. Much of the information is provided on the link above. Success rates are pretty high and many entries in the PDB have had their initial crystal hits there. I hope this helps, the center does not advertise much but works with a lot of laboratories. Cheers, Eddie. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu<http://hwi.buffalo.edu/> Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: [email protected]<mailto:[email protected]> [https://mail.google.com/mail/u/0/?ui=2&ik=62aec2015e&view=fimg&th=15b6914f7fdf6013&attid=0.1&disp=emb&attbid=ANGjdJ9g06QLo-D_EH41Fh-YwWWc3MjxJbO2-5rUPyw9xaU5whcTtrcEMgp6IRAWj-flG5nAubbpuMSCQvS1Drhckx7xSgFjo3hKVAHILDzuOysZW5m7BjsDwfq3SJc&sz=w656-h112&ats=1492181842461&rm=15b6914f7fdf6013&zw&atsh=1] Heisenberg was probably here! ________________________________ From: CCP4 bulletin board <[email protected]><mailto:[email protected]> on behalf of Elizabeth Diaz <[email protected]><mailto:[email protected]> Sent: Friday, April 14, 2017 9:06 AM To: [email protected]<mailto:[email protected]> Subject: [ccp4bb] Recommendations for Robotic Crystal Screening Services All, I am currently attempting to crystallize a peptide/protein complex and am wanting to know about robotic screening services that you would recommend. We have done some in house conditions with little luck, and want to broaden our search, but that would require going externally for screening. Do you have any recommendations for robotic crystal screening services, preferably in the United States? Thank you so much, Elizabeth Diaz University of Delaware
