Hi,
one word of caution with Metal fluoride complexes. Though they can
provide valuable inside in the reaction mechanism, even better than some
ATP-analogues, one has to be careful what metal to use to get the right
complex. See review below, which summarises the problems which can
occur, especially at lower resolution. Also when you have already other
metals in your crystallisation condition you might not get the complex
you want to.
Cheers
Christian
DOI:10.1002/anie.201606474 <https://doi.org/10.1002/anie.201606474>
Am 02.05.2017 um 19:42 schrieb Kevin Jude:
ADP-BeFx and ADP-AlFx (the latter mimics a dissociative transition
state). The fluoride compounds can be made by mixing BeCl2 or AlCl3
with excess NaF and then used at say a 3:1 excess over ADP. Be sure to
dissolve the chloride compounds in a fume hood!
Also ADP-VO4, which can be prepared as described in Goodno CC. Myosin
active-site trapping with vanadate ion. Meth Enzymol. 1982;85 Pt
B:116–23.
Best wishes
Kevin
On Tue, May 2, 2017 at 9:04 AM, Adriana Sene <adrianasen...@gmail.com
<mailto:adrianasen...@gmail.com>> wrote:
Hi
What are good ATP non hydrolyzable analog for crystallization?
best
Adriana
--
Kevin Jude, PhD
Research Specialist, Garcia Lab
Departments of Molecular & Cellular Physiology and Structural Biology
Stanford University School of Medicine
Beckman B177, 279 Campus Drive, Stanford CA 94305
Phone: (650) 723-6431 <tel:%28650%29%20723-6431>
