Hello,
I've seen BSA used to "block" membranes of centrifugal concentrators when proteins were sticking to them. It appeared to work (proteins could be concentrated with less loss), but I'm not sure how much BSA ends up in your sample. I can imagine the answer is "a bit" and therefore for some techniques this might not be appropriate. Dave Dr David C Briggs Hohenester Lab Department of Life Sciences Imperial College London UK http://about.me/david_briggs ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Ha Sin <[email protected]> Sent: 08 May 2017 13:32:44 To: [email protected] Subject: [ccp4bb] BSA as additive Dear CCP4BB members, Sorry about my non crystallography question. Does anyone know of a reference where BSA has been used as an "additive" in the protein concentration step to prevent aggregation of the main protein? I would appreciate your suggestions. Thank you, H.Sin
