Yes no images came through, however on looking at your crystallisation conditions for clues, is there anything in the protein prep that could have been carried through, even from the early stage buffers, or cell growth media? We once had a detergent in the cell lysis stage that was removed from all subsequent buffers (and protein put down 2 columns) but it made it through and produced density in the crystal structure.
Good luck with your search, Nicola ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Ian Clifton <[email protected]> Sent: 07 July 2017 14:23:36 To: [email protected] Subject: Re: [ccp4bb] Any suggestions? "Dr. Isabel De Moraes" <[email protected]> writes: > Any suggestions regarding to the positive densities? > Your pictures didn’t seem to make it as attachments, at least as received here. BW, -- Ian ◎
