Actually, you should try /increasing/ the protein concentration - a
lot. But be prepared to drop the precipitant concentration to almost
nothing (1 or 2% isn't "low").
To understand why, look at the phase diagram and what we assume about
vapour diffusion. (Which I'm assuming is what you're doing.)
On 12/07/2017 06:28, Vicky Tsirkone wrote:
Dear Patrick,
You may reduce the protein concentation, as well.
Another option could be the *streak seeding* by exploiting the drop of
your initial condition.
Good luck,
V.T.
On Mon, Jul 10, 2017 at 7:17 PM, Patrick Shaw Stewart
<[email protected] <mailto:[email protected]>> wrote:
Microseed them into two or three random screens.
Search for MMS and rMMS online.
Good luck
Patrick
On 10 July 2017 at 15:47, Liuqing Chen <[email protected]
<mailto:[email protected]>> wrote:
hello everyone!
I get a condition (10% w/v PEG 6000, 100mm HEPES PH7.0) in
which my protein grow small needle like crystals, how can i
optimize it to get bigger crystals? the attach is the
crystals figure.
thanks in advance
sincerely
Liuqing Chen
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