Hi,
often co-expressing and purifying proteins can help complex formation.
Best,
Francesca
On Tuesday, July 18, 2017 4:18 AM, El Sahili Abbas (Dr)
<[email protected]> wrote:
Hi,
This might indicate that the binding affinity of your complex is not high
enough for it to resist the gel filtration dilution. Maybe running a native
PAGE gel would allow you to visualise a complex.
Best
A
> On 18 Jul 2017, at 10:25, 高艺娜 <[email protected]> wrote:
>
> Hi all ,
>
> It has been reported the Negative stain EM of a protein A-B complex, but
> according to my gel filtration results (I purified A and B respectively for
> incubation) , I found that A could not bind to B, of course I tried different
> buffer condition with various pH value, even the binding condition only had
> 50 mm Kcl. Do you have any suggestion or methods that I can try to get the
> protein A-B complex?
>
> Any suggestion is welcome，
>
> Thank you all ,
>
> Best,
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